6SC9
dAb3/HOIP-RBR-HOIPIN-8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-06 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.127, 87.052, 241.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.900 - 2.470 |
R-factor | 0.2121 |
Rwork | 0.210 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | dAb3/HOIP-RBR apo structure |
RMSD bond length | 0.002 |
RMSD bond angle | 0.494 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.900 | 2.556 |
High resolution limit [Å] | 2.467 | 2.467 |
Rmerge | 0.049 | 0.701 |
Rmeas | 0.069 | 0.992 |
Number of reflections | 25600 | 2511 |
<I/σ(I)> | 5.57 | |
Completeness [%] | 99.8 | |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.280 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium sulfate, sodium chloride, HEPES |