6SC4
Gamma-Carbonic Anhydrase from the Haloarchaeon Halobacterium sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.28 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 362.640, 362.640, 362.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.20440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v3w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | 0.575 |
| Number of reflections | 62896 | 12673 |
| <I/σ(I)> | 18.8 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.05 M CdSO4, 0.8 M NaOAc |
