6SBS
YtrA from Sulfolobus acidocaldarius, a GntR-family transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 62 |
Unit cell lengths | 133.090, 133.090, 39.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.420 - 2.801 |
R-factor | 0.2525 |
Rwork | 0.249 |
R-free | 0.32080 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.420 | 2.870 |
High resolution limit [Å] | 2.801 | 2.801 |
Rmeas | 0.209 | |
Number of reflections | 10103 | 727 |
<I/σ(I)> | 8.88 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.01 | |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20 % (w/v) 229 PEG3350, 0.2 M sodium nitrate and 0.1 M Bis-Tris propane, pH 8.5 |