6SBP

Plant Cysteine Oxidase PCO5 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	68.910, 68.910, 123.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.727 - 1.910
R-factor	0.2055
Rwork	0.205
R-free	0.21880
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.743	2.210	1.960
High resolution limit [Å]	1.910	2.150	1.910
Rmerge		1.378
Rmeas	0.065	1.478	1.255
Rpim		0.403
Number of reflections	44135	16303	1720
<I/σ(I)>	21	2.1	2.4
Completeness [%]	99.8	99.7	100
Redundancy	12.8	13.3	13.5
CC(1/2)	1.000	0.757	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% PEG 3350, 0.2M Ammonium nitrate
1	VAPOR DIFFUSION, SITTING DROP		295	20% PEG 3350, 0.2M Ammonium nitrate