6SBP
Plant Cysteine Oxidase PCO5 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.910, 68.910, 123.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.727 - 1.910 |
R-factor | 0.2055 |
Rwork | 0.205 |
R-free | 0.21880 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.743 | 2.210 | 1.960 |
High resolution limit [Å] | 1.910 | 2.150 | 1.910 |
Rmerge | 1.378 | ||
Rmeas | 0.065 | 1.478 | 1.255 |
Rpim | 0.403 | ||
Number of reflections | 44135 | 16303 | 1720 |
<I/σ(I)> | 21 | 2.1 | 2.4 |
Completeness [%] | 99.8 | 99.7 | 100 |
Redundancy | 12.8 | 13.3 | 13.5 |
CC(1/2) | 1.000 | 0.757 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 3350, 0.2M Ammonium nitrate | |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 3350, 0.2M Ammonium nitrate |