6SBJ

X-ray structure of mus musculus Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) apo-form uuncomplexed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 1 21 1
Unit cell lengths	52.401, 69.959, 107.557
Unit cell angles	90.00, 92.25, 90.00

Refinement procedure

Resolution	42.650 - 2.220
R-factor	0.1962
Rwork	0.193
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6foh
RMSD bond length	0.010
RMSD bond angle	1.601
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.650	2.466
High resolution limit [Å]	2.217	2.217
Rmerge	0.251	0.958
Rmeas	0.298	1.138
Rpim	0.159	0.609
Number of reflections	26144	1316
<I/σ(I)>	4.6	1.4
Completeness [%]	89.2	45.6
Redundancy	3.44	3.38
CC(1/2)	0.963	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% (w/v) PEG 3350, 0.2 M MgCl2 and 0.1 M Bis/Tris pH 5.5