6SBB
Structure of type II terpene cyclase MstE from Scytonema (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.460, 48.580, 77.990 |
| Unit cell angles | 89.04, 99.97, 101.86 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2sqc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.049 | 0.549 |
| Number of reflections | 46089 | 5260 |
| <I/σ(I)> | 9.4 | 2 |
| Completeness [%] | 92.2 | 92.5 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM TRIS, 25% PEG 8000 |
