6SB3
CryoEM structure of murine perforin-2 ectodomain in a pre-pore form
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Software | RELION,SerialEM,Gctf |
Symmetry | C16 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |