6SAX
Chromophore binding domain of bacteriophytochrome linked diguanylyl cyclase from Idiomarina species A28L (Pr-state monomer).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-08 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.873130 |
Spacegroup name | P 61 |
Unit cell lengths | 149.960, 149.960, 77.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.894 - 2.400 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5llw |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.894 | 53.894 | 2.500 |
High resolution limit [Å] | 2.400 | 20.000 | 2.400 |
Rmerge | 0.202 | 0.047 | 1.618 |
Rmeas | 0.213 | 0.052 | 1.702 |
Total number of observations | 395067 | ||
Number of reflections | 38887 | 63 | 4453 |
<I/σ(I)> | 9.69 | 34.71 | 1.57 |
Completeness [%] | 99.7 | 80.8 | 99.5 |
Redundancy | 10.159 | 7.46 | 10.418 |
CC(1/2) | 0.996 | 0.996 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.24 M sodium malonate pH 7.0, 20 % PEG 3,350 |