6SAU
Structural and functional characterisation of three novel fungal amylases with enhanced stability and pH tolerance.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.877, 61.966, 70.395 |
| Unit cell angles | 79.33, 82.88, 67.99 |
Refinement procedure
| Resolution | 33.130 - 1.350 |
| R-factor | 0.1146 |
| Rwork | 0.113 |
| R-free | 0.15030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.249 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 163777 | 3628 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 85.3 | 38.2 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 12% PEG 3350 0.2 M NaNO3, CAPS pH 11.0 |
