6SAN
SALSA / DMBT1 / GP340 SRCR domain 8 soaked in calcium and magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-10-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.235, 46.643, 93.628 |
Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
Resolution | 30.950 - 1.360 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sa5 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.873 |
Data scaling software | autoPROC (1.0.5) |
Phasing software | MOLREP (CCP4Interface 7.0.056) |
Refinement software | PHENIX (dev_3126) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.640 | 1.390 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmeas | 0.074 | 0.801 |
Number of reflections | 49168 | 4786 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 98.3 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 50 % of 0.2 M MgCl2 hexahydrate, 20 % (v/v) isopropanol, 0.1 M HEPES, pH: 7.5. After crystal-formation, crystals were soaked in 10 mM MgCl2 and 10 mM CaCl2. |