6S9U
Crystal structure of sucrose 6F-phosphate phosphorylase from Ilumatobacter coccineus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.434, 92.054, 179.971 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.027 - 2.050 |
R-factor | 0.1674 |
Rwork | 0.166 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc1_3161) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.030 | 46.030 | 2.110 |
High resolution limit [Å] | 2.050 | 8.940 | 2.050 |
Rmerge | 0.106 | 0.047 | 0.829 |
Rmeas | 0.118 | 0.054 | 0.925 |
Rpim | 0.051 | 0.024 | 0.405 |
Total number of observations | 169930 | 1983 | 13050 |
Number of reflections | 33373 | 463 | 2567 |
<I/σ(I)> | 9.7 | 22.5 | 2.1 |
Completeness [%] | 99.6 | 98.1 | 100 |
Redundancy | 5.1 | 4.3 | 5.1 |
CC(1/2) | 0.995 | 0.997 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG 3350, 200 mM Na/K phosphate, 100 mM Bis-Tris propane |