6S9T
Dimerization domain of Xenopus laevis LDB1 in complex with darpin 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978860 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.217, 70.217, 122.257 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.150 - 2.050 |
| R-factor | 0.2206 |
| Rwork | 0.218 |
| R-free | 0.27930 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.127 | |
| Rmeas | 0.129 | |
| Rpim | 0.021 | |
| Number of reflections | 22528 | 2198 |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 39.5 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M TrisHCL, pH 7.0 32% PEG200 0.05 M Li2SO4 |
