6S9S
Dimerization domain of Xenopus laevis LDB1 in complex with darpin 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.980040 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.509, 117.117, 93.971 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 2.200 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.28010 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.632 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.094 | |
| Rmeas | 0.098 | |
| Rpim | 0.027 | |
| Number of reflections | 22606 | 223 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.1 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M TRIS, pH 8.5 20% PEG 8K 0.2 M Li2SO4 |
