6S9M
Designed Armadillo Repeat protein Lock2 fused to target peptide KRKRKAKITW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000041 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.853, 85.474, 193.970 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 48.200 - 2.000 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.948 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.200 | 48.200 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.112 | 0.024 | 0.871 |
| Rmeas | 0.133 | 0.028 | 1.038 |
| Total number of observations | 793257 | ||
| Number of reflections | 228033 | 2591 | 16882 |
| <I/σ(I)> | 10.55 | 40.68 | 1.72 |
| Completeness [%] | 96.3 | 97.9 | 96 |
| Redundancy | 3.479 | 3.676 | 3.423 |
| CC(1/2) | 0.996 | 0.999 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 22.5 % PEG smear medium, 0.1 M CaCl2, 0.1 M MgCl2,0.1 M Pipes pH 7 |
