6S9A
Artificial GTPase-BSE dimer of human Dynamin1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.2825 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.693, 69.546, 74.277 |
Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
Resolution | 48.730 - 1.860 |
R-factor | 0.2194 |
Rwork | 0.219 |
R-free | 0.25450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d3q |
RMSD bond length | 0.012 |
RMSD bond angle | 1.442 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.730 | 1.970 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 55429 | 17351 |
<I/σ(I)> | 9.02 | |
Completeness [%] | 98.5 | 98.3 |
Redundancy | 3.49 | 3.47 |
CC(1/2) | 0.999 | 0.216 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Tris-HCl, 0.25M KBr, 30% (w/v) PEG MME 2000 |