6S99
Dimethylated fusion protein of RSL and trimeric coiled coil in complex with cucurbit[7]uril
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 51.219, 51.242, 111.282 |
Unit cell angles | 76.80, 89.88, 60.05 |
Refinement procedure
Resolution | 42.842 - 2.649 |
R-factor | 0.2262 |
Rwork | 0.225 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.364 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.842 | 2.695 |
High resolution limit [Å] | 2.649 | 2.649 |
Rmerge | 0.056 | 0.313 |
Rpim | 0.035 | 0.190 |
Number of reflections | 26787 | 1383 |
<I/σ(I)> | 7.8 | 2.7 |
Completeness [%] | 97.4 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M TRIS HCL pH 8.5, 20% PEG8000, 0.2 M Magnesium Chloride, 0.0013 M cucurbit[7]uril |