6S8Z

Elongation Factor P from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	P 1 21 1
Unit cell lengths	51.201, 38.163, 55.356
Unit cell angles	90.00, 93.09, 90.00

Refinement procedure

Resolution	38.580 - 2.200
Rwork	0.206
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oyy
RMSD bond length	0.013
RMSD bond angle	1.721
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.580	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.129	2.355
Rmeas	0.145	2.631
Rpim	0.065	1.158
Number of reflections	10932	1081
<I/σ(I)>	7.98	0.63
Completeness [%]	98.6	98.9
Redundancy	4.9	5.1
CC(1/2)	0.996	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Sodium acetate 0.1 M HEPES pH 7.5 22 %(w/v) PEG 4000