6S8X

Crystal structure of the Rab-binding domain of FIP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	62.538, 68.430, 172.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.020 - 2.290
R-factor	0.2473
Rwork	0.246
R-free	0.27390
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.778
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.160	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.111	0.872
Rmeas	0.126	1.048
Rpim	0.059	0.483
Number of reflections	16934	1611
<I/σ(I)>	8.8	1.7
Completeness [%]	99.4	99.3
Redundancy	4.3	4.3
CC(1/2)		0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.01M cobat chloride 0.1M NaOAc, pH 4.7 1M 1,6 hexanediol