6S8T
Structure of the ICAM-1-binding PfEMP1 IT4var13 DBLbeta domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.302, 43.451, 87.129 |
| Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
| Resolution | 46.727 - 2.170 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.727 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 26322 | 1229 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Magnesium chloride hexahydrate 0.1M BIS-Tris pH 5.5 25% (w/v) PEG 3350 |
