6S8C
Post-fusion conformation of the envelope protein of tick-borne encephalitis virus with longer stem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99 |
| Spacegroup name | H 3 |
| Unit cell lengths | 169.435, 169.435, 123.567 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.910 - 2.570 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.910 | 48.910 | 2.670 |
| High resolution limit [Å] | 2.570 | 9.610 | 2.570 |
| Rmerge | 0.115 | 0.037 | 1.167 |
| Rmeas | 0.136 | 0.044 | 1.382 |
| Rpim | 0.072 | 0.023 | 0.731 |
| Total number of observations | 147146 | 2764 | 15174 |
| Number of reflections | 41959 | 793 | 4356 |
| <I/σ(I)> | 7.9 | 26.2 | 1.1 |
| Completeness [%] | 99.5 | 98.1 | 97.5 |
| Redundancy | 3.5 | 3.5 | 3.5 |
| CC(1/2) | 0.994 | 0.997 | 0.290 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 19% isopropanol, 100mM tris-sodium citrate pH 5.6, 19% PEG 4000, 5% glycerol |
