6S7V
Lipoteichoic acids flippase LtaA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 289 |
Detector technology | PIXEL |
Collection date | 2018-06-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.390, 162.470, 191.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.996 - 3.301 |
R-factor | 0.2714 |
Rwork | 0.271 |
R-free | 0.28940 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.996 | 19.996 | 3.430 |
High resolution limit [Å] | 3.300 | 13.300 | 3.320 |
Rmerge | 0.132 | 0.050 | 1.499 |
Rmeas | 0.135 | 0.052 | 1.537 |
Number of reflections | 10414 | 350 | 747 |
<I/σ(I)> | 12.76 | 56.16 | 3.41 |
Completeness [%] | 83.9 | 97.2 | 34.6 |
Redundancy | 14 | 21.266 | 14 |
CC(1/2) | 0.999 | 0.999 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.5 | 289 | Glycine: MgAcetate:PEG300 |