6S7N
Crystal structure of orthorhombic lysozyme grown at pH 5.5 with a 26% of solvent content
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.037, 51.699, 61.531 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.846 - 1.200 |
R-factor | 0.1605 |
Rwork | 0.160 |
R-free | 0.17410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f1m |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.850 | 19.850 | 1.220 |
High resolution limit [Å] | 1.200 | 6.570 | 1.200 |
Rmerge | 0.040 | 0.032 | 0.695 |
Rmeas | 0.046 | 0.038 | 0.810 |
Rpim | 0.022 | 0.019 | 0.409 |
Total number of observations | 121637 | 746 | 5527 |
Number of reflections | 30547 | 216 | 1460 |
<I/σ(I)> | 13.5 | 37.5 | 1.9 |
Completeness [%] | 99.5 | 93.9 | 99.9 |
Redundancy | 4 | 3.5 | 3.8 |
CC(1/2) | 0.999 | 0.998 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2 M sodium chloride, 5mM lithium sulphate, 0.1M sodium acetate |