6S5Y
Structure of tandemly arrayed consecutive Rib domains (Rib2R) from Group B Streptococcal species Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.825, 380.854, 37.853 |
| Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
| Resolution | 38.090 - 2.300 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.090 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.150 | |
| Number of reflections | 47115 | 4792 |
| <I/σ(I)> | 8.3 | 0.5 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.999 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 279 | 3M ammonium sulphate |
