6S5Y

Structure of tandemly arrayed consecutive Rib domains (Rib2R) from Group B Streptococcal species Streptococcus agalactiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	37.825, 380.854, 37.853
Unit cell angles	90.00, 91.60, 90.00

Refinement procedure

Resolution	38.090 - 2.300
R-factor	0.235
Rwork	0.234
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.090
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.090	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.150
Number of reflections	47115	4792
<I/σ(I)>	8.3	0.5
Completeness [%]	99.6	99.6
Redundancy	6.9	7
CC(1/2)	0.999	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		279	3M ammonium sulphate