6S5W

Structure of Rib domain 'Rib Long' from Lactobacillus acidophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	43.370, 147.810, 56.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.946 - 1.070
R-factor	0.1487
Rwork	0.147
R-free	0.17770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.881
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.950	1.090
High resolution limit [Å]	1.070	1.070
Rmerge	0.065
Rmeas	0.070
Rpim	0.026
Number of reflections	78337	3096
<I/σ(I)>	13.2	1.1
Completeness [%]	97.3
Redundancy	6.9	3.6
CC(1/2)	0.999	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M Ammonium Sulphate, 30% PEG 8000