6S52
The crystal structure of glycogen phosphorylase in complex with 14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION SUPERNOVA |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2018-05-29 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5419 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.128, 128.128, 115.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.750 - 2.370 |
R-factor | 0.14858 |
Rwork | 0.146 |
R-free | 0.20385 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.683 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.750 | 2.460 |
High resolution limit [Å] | 2.370 | 2.370 |
Number of reflections | 35891 | 3175 |
<I/σ(I)> | 13.3 | 2 |
Completeness [%] | 91.1 | 78.1 |
Redundancy | 5.2 | 3.7 |
CC(1/2) | 0.995 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer, pH 6.7 |