6S51
The crystal structure of glycogen phosphorylase in complex with 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION SUPERNOVA |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2018-05-29 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5419 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.077, 128.077, 115.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.740 - 2.370 |
R-factor | 0.15532 |
Rwork | 0.153 |
R-free | 0.20502 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.740 | 2.460 |
High resolution limit [Å] | 2.370 | 2.370 |
Number of reflections | 35501 | 3121 |
<I/σ(I)> | 13.9 | 2.3 |
Completeness [%] | 90.5 | 77.2 |
Redundancy | 4.9 | 3.6 |
CC(1/2) | 0.996 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer, pH 6.7 |