6S4O

The crystal structure of glycogen phosphorylase in complex with 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION SUPERNOVA
Temperature [K]	293
Detector technology	CCD
Collection date	2018-05-29
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5419
Spacegroup name	P 43 21 2
Unit cell lengths	128.207, 128.207, 115.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.700 - 2.350
R-factor	0.15528
Rwork	0.153
R-free	0.20741
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.458
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.700	2.440
High resolution limit [Å]	2.350	2.350
Number of reflections	37798	3389
<I/σ(I)>	13.7	2.4
Completeness [%]	93.2	80.9
Redundancy	5.3	3.7
CC(1/2)	0.994	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM BES buffer, pH 6.7