6S4C
Crystal Structure of the vWFA2 subdomain of type VII collagen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 123.034, 123.034, 63.749 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.520 - 2.000 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4igi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.755 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.520 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.139 | 0.709 |
| Number of reflections | 12575 | 923 |
| <I/σ(I)> | 6.9 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1.8 M mangesium sulphate, 0.1 M sodium acetate |
