6S3P
Crystal structure of helicase Pif1 from Thermus oshimai in complex with (dT)18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.850, 64.583, 144.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.730 - 1.926 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ftd |
RMSD bond length | 0.009 |
RMSD bond angle | 0.926 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.730 | 1.995 |
High resolution limit [Å] | 1.926 | 1.926 |
Rmerge | 0.056 | 0.463 |
Rmeas | 0.060 | 0.509 |
Rpim | 0.023 | 0.208 |
Number of reflections | 38861 | 2388 |
<I/σ(I)> | 21.62 | 4.01 |
Completeness [%] | 88.1 | 55.48 |
Redundancy | 6.9 | 5.4 |
CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Tris-HCl 0.1M NaF 0.03M NaI 0.03M NaBr 0.03M MES 0.03M PEG 4K 10% Glycerol 20% |