6S3E

Crystal structure of helicase Pif1 from Thermus oshimai in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	74.268, 59.408, 117.150
Unit cell angles	90.00, 91.82, 90.00

Refinement procedure

Resolution	36.710 - 3.787
R-factor	0.2396
Rwork	0.237
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftd
RMSD bond length	0.004
RMSD bond angle	1.044
Data reduction software	XDS (Nov 1, 2016, built on 20170215)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.710	3.920
High resolution limit [Å]	3.780	3.780
Rmerge	0.141	1.121
Rmeas	0.153	1.210
Rpim	0.058	0.453
Number of reflections	10098	738
<I/σ(I)>	12.2	2.45
Completeness [%]	96.7	70.4
Redundancy	7.2	6.9
CC(1/2)	0.996	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	Sodium Hepes-MOPS 0.1M Ethylene glycol 1.25% PEG 4000 20% Glycerol 20%