6S34
Zinc free, dimeric human insulin determined to 1.35 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | I 21 3 |
Unit cell lengths | 78.170, 78.170, 78.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.080 - 1.350 |
Rwork | 0.161 |
R-free | 0.17740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b2d |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.080 | 1.398 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.058 | 1.610 |
Rmeas | 0.060 | 1.656 |
Rpim | 0.014 | 0.389 |
Number of reflections | 17613 | 1749 |
<I/σ(I)> | 28.21 | 1.75 |
Completeness [%] | 100.0 | 99.94 |
Redundancy | 19.5 | 18 |
CC(1/2) | 1.000 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Sodium Citrate, 0.1 M Sodium Hepes, pH 7.5, 20 % isopropanol |