6S2Y

Water-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum with Chlorophyll-b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-07-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	73.310, 82.900, 122.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.630 - 2.300
R-factor	0.2451
Rwork	0.243
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dre
RMSD bond length	0.010
RMSD bond angle	2.384
Data reduction software	XDS (VERSION Mar 15, 2019)
Data scaling software	XSCALE (VERSION Mar 15, 2019)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.630	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.078
Number of reflections	33323	2427
<I/σ(I)>	12.31	3.03
Completeness [%]	98.7	99.8
Redundancy	4.62	4.76
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M Na/K Phosphate, 3.0M Ammonium sulfate, 4.7% Sucrose