6S2X
Crystal structure of the Legionella pneumophila ChiA C-terminal domain
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.370, 56.640, 128.960 |
Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
Resolution | 64.339 - 1.710 |
Rwork | 0.146 |
R-free | 0.16800 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.472 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 64.340 | 1.940 | 1.750 |
High resolution limit [Å] | 1.710 | 1.890 | 1.710 |
Rmeas | 0.066 | 3.990 | 0.712 |
Rpim | 0.025 | 1.159 | 0.269 |
Number of reflections | 75905 | 2309 | 5555 |
<I/σ(I)> | 19.2 | 1.4 | 2.8 |
Completeness [%] | 99.9 | 55 | 99.9 |
Redundancy | 6.8 | 11.4 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 45% (v/v) 2-Methyl-2,4-pentanediol | |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 45% (v/v) 2-Methyl-2,4-pentanediol |