6S2L
FMDV 3D polymerase crystallized in presence of (F)uridylylated VPg peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97692 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.850, 93.850, 121.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.230 - 2.300 |
| R-factor | 0.20011 |
| Rwork | 0.197 |
| R-free | 0.25203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u09 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.230 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.777 |
| Rpim | 0.039 | 0.319 |
| Number of reflections | 24747 | 3536 |
| <I/σ(I)> | 12.7 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M ammonium acetate, 30% PEG 4K, 0.1M MES pH 6.0, 4 butyrolactone |
