6RYK
Crystal structure of the ParB-like protein PadC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-27 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87290 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.850, 42.440, 49.530 |
| Unit cell angles | 108.54, 99.15, 89.28 |
Refinement procedure
| Resolution | 36.740 - 1.700 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.22650 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.236 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.328 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.980 |
| Number of reflections | 29970 | 2956 |
| <I/σ(I)> | 11.03 | 1.11 |
| Completeness [%] | 97.1 | 96.07 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.990 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M NaCl, 20 % (w/v) PEG 3350 |
