6RY6
Crystal structure of Dfg5 from Chaetomium thermophilum in complex with glucosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-26 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.452, 55.084, 80.506 |
Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
Resolution | 40.250 - 1.300 |
R-factor | 0.114 |
Rwork | 0.113 |
R-free | 0.12740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.250 | 1.346 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.020 | 0.140 |
Rmeas | 0.035 | |
Number of reflections | 86433 | 8481 |
<I/σ(I)> | 15.54 | 4.51 |
Completeness [%] | 96.3 | 94.52 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.999 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | Ammoniumiodide, PEG3350, Calcium acetate, MES, PEG 8000 |