6RY3

Structure of the WUS homeodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 41 21 2
Unit cell lengths	43.120, 43.120, 82.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.166 - 1.374
R-factor	0.1859
Rwork	0.184
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hdd
RMSD bond length	0.010
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.170	1.423
High resolution limit [Å]	1.374	1.374
Rmerge	0.038
Rpim	0.008	0.030
Number of reflections	16816	1628
<I/σ(I)>	61.11
Completeness [%]	99.8
Redundancy	24.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.5 M ammonium sulfate 0.05 M MES (pH 6.0) 0.01 M magnesium acetate