6RY2
Crystal structure of Dfg5 from Chaetomium thermophilum in complex with alpha-1,2-mannobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.715, 54.863, 80.128 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 41.856 - 1.300 |
| R-factor | 0.1065 |
| Rwork | 0.105 |
| R-free | 0.12910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.930 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.116 |
| Rmeas | 0.019 | |
| Number of reflections | 84221 | 8154 |
| <I/σ(I)> | 8.63 | 6.01 |
| Completeness [%] | 94.3 | 91.56 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.990 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | Ammoniumiodide, PEG3350, Calcium acetate, MES, PEG 8000 |
