6RXF
Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.508, 73.118, 88.987 |
| Unit cell angles | 71.30, 72.99, 78.99 |
Refinement procedure
| Resolution | 52.774 - 2.397 |
| Rwork | 0.182 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rxd |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.860 | 2.480 |
| High resolution limit [Å] | 2.397 | 2.397 |
| Rmerge | 0.233 | |
| Number of reflections | 46820 | 4365 |
| <I/σ(I)> | 2.3 | 1.5 |
| Completeness [%] | 94.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M MES, 0.01 M zinc chloride, 18 % PEG 6000 |
