6RWC

crystal structure of chicken beta-microseminoprotein-like 3 (MSMB3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97903
Spacegroup name	P 21 21 2
Unit cell lengths	47.176, 101.047, 36.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.747 - 2.143
R-factor	0.2218
Rwork	0.221
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ix0
RMSD bond length	0.002
RMSD bond angle	0.420
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.750	2.180
High resolution limit [Å]	2.140	2.140
Rmerge	0.082	0.536
Number of reflections	10095	446
<I/σ(I)>	12	1.6
Completeness [%]	98.8	91.6
Redundancy	5.6
CC(1/2)	0.998	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.7	293	Phosphate/citrate buffer, Li2SO4, PEG1000