6RW3

The molecular basis for sugar import in malaria parasites.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2018-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.971997
Spacegroup name	P 1 21 1
Unit cell lengths	73.740, 189.450, 136.890
Unit cell angles	90.00, 96.43, 90.00

Refinement procedure

Resolution	21.850 - 3.650
R-factor	0.2732
Rwork	0.273
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zw9
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Rosetta
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.850	3.800
High resolution limit [Å]	3.650	3.650
Rmerge	0.285
Number of reflections	41268	4095
<I/σ(I)>	10
Completeness [%]	99.4
Redundancy	12.4
CC(1/2)	0.990	0.210

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	MES, Magnesium Chloride,PEG 300, NG
1	VAPOR DIFFUSION	6.5	293	MES, Magnesium Chloride,PEG 300, NG