6RV5
X-ray structure of the levansucrase from Erwinia tasmaniensis in complex with levanbiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-13 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 127.886, 127.886, 58.268 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.260 - 1.580 |
R-factor | 0.12623 |
Rwork | 0.146 |
R-free | 0.19290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6frw |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.420 | 1.640 |
High resolution limit [Å] | 1.450 | 1.580 |
Rmerge | 0.027 | 0.287 |
Rmeas | 0.038 | 0.406 |
Rpim | 0.027 | 0.287 |
Number of reflections | 66162 | 6517 |
<I/σ(I)> | 2.04 | |
Completeness [%] | 99.5 | |
Redundancy | 1.3 | |
CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 28% Glycerol, 14% PEG 4000, 1.5mM Manganese(II) chloride tetrahydrate, 1.5 mM Cobalt(II) chloride hexahydrate , 1.5 mM Nickel(II) chloride hexahydrate, 1.5 mM Zinc acetate dihydrate crystals soaked in Sucrose 500 mM |