6RV1
human Alanine:Glyoxylate Aminotransferase major allele (AGT-Ma)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.333, 89.333, 140.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.000 - 3.000 |
| R-factor | 0.2747 |
| Rwork | 0.273 |
| R-free | 0.31640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.020 | 47.020 | 3.180 |
| High resolution limit [Å] | 3.000 | 9.000 | 3.000 |
| Rmerge | 0.098 | 0.030 | 2.391 |
| Rmeas | 0.103 | 0.032 | 2.486 |
| Rpim | 0.029 | 0.010 | 0.675 |
| Total number of observations | 150101 | 5165 | 25153 |
| Number of reflections | 12014 | 524 | 1880 |
| <I/σ(I)> | 14.5 | 59.2 | 1.2 |
| Completeness [%] | 99.9 | 99.5 | 99.9 |
| Redundancy | 12.5 | 9.9 | 13.4 |
| CC(1/2) | 0.999 | 0.999 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 10-12% PEG 6K; 5% 2-methyl-2,4-pentanediol (MPD); 0.1M MES pH 6.5 |
