6RUX

P46, an immunodominant surface protein from Mycoplasma hyopneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	136.178, 136.178, 139.332
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.000 - 2.500
Rwork	0.209
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rqg
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.030
Number of reflections	52027	5152
<I/σ(I)>	35.42
Completeness [%]	60.0
Redundancy	9.3
CC(1/2)	0.990	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris pH 8.5 0.2 M Sodium Acetate 32% PEG 4000 3% Maltose