6RUT
Mycoplasma Genitalium Heterodimer Nap Complex (P140-P110 globular)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-05-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97897 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 151.000, 157.280, 192.370 |
Unit cell angles | 90.00, 93.81, 90.00 |
Refinement procedure
Resolution | 121.660 - 2.650 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r3t |
RMSD bond length | 0.010 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 157.280 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 189359 | 784 |
<I/σ(I)> | 6.6 | |
Completeness [%] | 73.3 | |
Redundancy | 6.8 | |
CC(1/2) | 0.873 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 23% PEG MME 4% PGA 0.1M Sodium cacodylate pH 6.5 |