6RUL
Crystal structure of GFP-LAMA-F98 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97797 |
Spacegroup name | P 62 |
Unit cell lengths | 86.610, 86.610, 73.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.305 - 2.200 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uii 5h8d |
RMSD bond length | 0.008 |
RMSD bond angle | 1.068 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.058 | 0.453 |
Number of reflections | 15911 | 1970 |
<I/σ(I)> | 22.62 | 5.32 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.1 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M MES pH 6.0, 30% (v/v) PEG 600, 5% (w/v) PEG 1000, 10% (v/v) glycerol |