6RU3

Crystal structure of the FP specific nanobody hFPNb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	H 3 2
Unit cell lengths	65.120, 65.120, 169.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.850 - 1.260
R-factor	0.189
Rwork	0.188
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.153
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.420	1.260
High resolution limit [Å]	1.230	1.230
Number of reflections	39873	2460
<I/σ(I)>	32.3
Completeness [%]	98.3	83.4
Redundancy	34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	13.1 % (v/v) MPD, 13.1 % PEG1000 (w/v) 13.1 % (w/v) PEG3350, 100 mM Tris Bicine pH 8.5, 33 mM CaCl2, 33 mM MgCl2, 4 % (v/v) formamide, 114 mM FOS-Choline 8