6RTR
Piperideine-6-carboxylate dehydrogenase from Streptomyces clavuligerus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9764 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.720, 130.370, 157.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.250 - 1.550 |
| R-factor | 0.16472 |
| Rwork | 0.164 |
| R-free | 0.18627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jg7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.711 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmeas | 0.071 | 0.764 |
| Number of reflections | 161305 | 49255 |
| <I/σ(I)> | 17.72 | 2.89 |
| Completeness [%] | 98.5 | 97.4 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate pH 5, 2.2 M ammonium sulphate, 0.2 M lithium acetate |
