6RTH
Crystal structure of the ligand-free glycosyltransferase domain from the YGT toxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.178, 161.737, 162.971 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.400 |
R-factor | 0.228 |
Rwork | 0.227 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rtg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.670 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.473 | 1.508 |
Number of reflections | 17726 | 3590 |
<I/σ(I)> | 7 | 2.2 |
Completeness [%] | 99.7 | |
Redundancy | 13 | 12.2 |
CC(1/2) | 0.969 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM sodium formate, 20% PEG 3350 |