6RSX
Regulatory Subunit of cAMP-dependant Protein Kinase A from Euglena gracilis at 1.6 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 |
Unit cell lengths | 80.586, 80.586, 110.755 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 69.790 - 1.600 |
R-factor | 0.193489922461 |
Rwork | 0.192 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rgs |
RMSD bond length | 0.011 |
RMSD bond angle | 1.084 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.790 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.073 | 0.081 |
Rmeas | 0.085 | |
Number of reflections | 52962 | 7528 |
<I/σ(I)> | 14.33 | |
Completeness [%] | 99.6 | |
Redundancy | 10.1 | |
CC(1/2) | 0.999 | 0.483 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 1) 50mM MES pH 6.0 , 4% MPD , 80mM Ammonium sulfate , 18% PEG 8000 2) 50mM MES pH 6.0, 20% PEG 3350 3) 50mM MES pH 6.0 , 4% MPD , 0.2M Ammonium sulfate , 20% PEG 3350 |